University of South Florida Showcase

This dataset contains measurements of concentrations of fecal indicator bacteria (Escherichia coli and enterococci) and microbial source tracking (MST) markers (HF183, Rum2Bac, GFD, DG37, CowM3, and GenBac), and detections of culturable E. coli that carry the sewage-associated H8 gene. This data also includes environmental parameters recorded during each sampling event, including estimations of tide and precipitation, measurements of water temperature, dissolved oxygen, pH, salinity, and turbidity. Surface water was sampled in five different water bodies (Bullfrog Creek, Frenchmans Creek, Hamilton Creek, Northshore Park, and Salt Creek) that discharge directly or indirectly to Tampa Bay in Hillsborough and Pinellas, Florida counties. Samples were collected at several sites in each water body on a monthly basis from July 2022 to June 2024 to identify major sources of fecal pollution. QPCR MST data were also used to estimate human health risk ascribed to primary contact recreational exposure by QMRA.

NEON staff collected pinnae (ear tissue) samples from Peromyscus leucopus (N = 55) and P. maniculatus (N = 38) in 2022 from two sites (see the Animals and Study Site section in the main text). The samples were used to obtain RNA (see RNA extraction in the main text) and measure immune gene expression (see the Droplet Digital Polymerase Chain Reaction (ddPCR) section in the main text).

This dataset contains integrated red tide (Karenia brevis harmful algal blooms) maps for the West Florida Shelf (24N – 31N, 87W – 81W) every month between 2003 and 2019. The integration is over in situ water sample analysis (which provides K. brevis cell concentration) and satellite data (which provides more frequent and spatial coverage than in situ data) using a practical approach. In situ cell counts data were obtained from a database maintained by the Florida Fish & Wildlife Conservation Commission (https://myfwc.com/research/redtide/monitoring/database/), and satellite data were collected by the Moderate Resolution Imaging Spectroradiometer (MODIS) onboard the Aqua satellite, available through NASA’s OB.DAAC (https://oceancolor.gsfc.nasa.gov). They were integrated to generate monthly maps of K. brevis bloom spatial extent and frequency. Specifically, the maps are expressed as bloom occurrence frequency (BOF) and bloom intensity (BI). The boundary of bloom locations represents the bloom footprint. Both maps are in imagery format (color coded in png files, MODIS_KB_bloom_WFS_images.zip) and binary format (floating point Geotiff files, MODIS_KB_bloom_WFS_binary.zip).

This dataset contains measurements of concentrations of MST marker HF183 and fecal indicator microorganisms (fecal coliforms and enterococci). Water samples were collected just outside the river plume in three rivers that impact a Costa Rican beach during the rainy and dry season. Microorganisms were filtered from a 500-mL grab sample onto a 0.45-µm, mixed cellulose ester. Enterococci and fecal coliforms were cultured.HF183 was quantified from nucleic acid using quantitative PCR. We examined microbiological water quality in three rivers that impact a Costa Rican beach.

The objective of this study is to quantify PFASs in sediment and fishes collected from Tampa Bay to further estimate human health risks from dietary exposures. Sediment (n = 17) and fish (24 species, n = 140) were collected throughout Tampa Bay in 2020 and 2021 and analyzed for 25 PFAS compounds. Concentrations of PFASs in sediments and edible tissues of fish ranged from 36.8 to 2,990 ng kg-1 (dry weight) and 307 to 33,600 ng kg-1 (wet weight), respectively. Generally, levels were highest in Old Tampa Bay and decreased south towards the Gulf of Mexico. Profiles in both matrices were generally dominated by perfluorooctane sulfonic acid (PFOS) with variations by location.

This dataset contains measurements of fecal indicator bacteria (Escherichia coli and enterococci), microbial source tracking markers (HF183 and GFD), nutrients, and environmental parameters from two freshwater Florida streams. Water and sediment was collected over a 26-month period. Fecal indicator bacteria were cultured from water and sediment, microbial source tracking markers were assessed in water by qPCR, and nutrients were measured in water and sediment.

This data supports the findings in the manuscript titled "Predicting pH at in-situ temperature for aquatic environments". Experiments were performed to analyze how the pH of seawater changes with changing temperature conditions (∆pH/∆T) across a range of salinities. Data was collected by measuring pH spectrophotometrically with simultaneous temperature measurements via a mini surface temperature probe. The total alkalinity (AT) of the seawater sample and salinity were measured for each sample to fully define the marine CO2 system. ∆pH/∆T was determined for a pH (at 25°C) range of 7.2–8.2, a temperature range of 15–40°C and salinities 10.1, 20.2, 30.2, and 36.1.

This file includes the microbial community composition, community change, and predicted function for the paper titled "Simultaneous N and P removal in sequencing batch biofilm reactors (SBBRs) with sphalerite-oyster shell media."

This dataset collects a record of physical and chemical observations made by two uncrewed sailing vehicles (Saildrone 1038 and Saildrone 1039) in the South Indian Ocean during 2022 and 2023. SD1038 collected data near 15°E and between latitudes 35°S and 50°S during July 19-26, 2022. SD1039 made observations within the Subantarctic Zone (37-47°S, 20-45°E) between September 1, 2022 until February 24, 2023. The local atmospheric and surface ocean parameters measured are listed below: -- Atmospheric measurements: temperature, pressure, humidity. -- Seawater measurements: temperature, pressure, conductivity, salinity. -- Carbon measurements: fCO2, xCO2, and pCO2 in atmosphere and seawater. -- Chlorophyll measurements: concentration. -- Oxygen measurements: concentration, saturation, ratio of O2 in water to air. -- Wind measurements: eastward, northward, and downward speed, plus gusts and direction. -- Wave measurements: significant wave height and dominant wave period. -- Irradiation measurements (SD1039 only): longwave, shortwave, and PAR. -- Current velocity measurements (SD1039 only): eastwards, northwards, and upwards, down to 102m. -- CCMP wind estimates (hourly only): collocated to Saildrone time and location. -- Directions of large eddies transitted (SD1039 hourly only): based on AVISO eddy database. Four files included: -- SD1038_1min.nc (SD1038’s raw data at 1-minute timesteps with frequent gaps) -- SD1039_1min.nc (SD1039’s raw data at 1-minute timesteps with frequent gaps) -- SD1038_hrly.nc (SD1038’s hourly-averaged data, plus eddies and CCMP winds) -- SD1039_hrly.nc (SD1039’s hourly-averaged data, plus eddies and CCMP winds) 1-minute files contain the “raw” data, at all times it was collected. Because many variables were only sampled once or a few times an hour, these files include frequent gaps. Hourly files were made from the 1-minute data, averaging whatever data exists within each hour, such that few gaps in the data exist for most variables.

Numerical modeling simulation data for Lepas project