Radula stl files

Published: 1 May 2023| Version 1 | DOI: 10.17632/d5p97fcx3s.1
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Description

Specimens were scanned using single propagation distance tomography at the European Synchrotron Radiation Facility (ESRF) in Grenoble, France using the ID19 microtomography beamline. Every specimen used the following scan parameters: 2999 projections collected for a 360° rotation; 0.1 ms exposure time; 360 nm voxel size; 20 mm sample-to-detector distance; x-ray energy of 26.5 keV. Specimen preparation for scanning electron microscopy (SEM) was the same as for synchrotron preparation except that radulae were mounted on stubs with double-sided conductive tape, and coated with Au-Pd for imaging with a Philips XL 30 scanning electron microscope at the Museo Argentino de Ciencias Naturales. Synchrotron *.tif stacks were reconstructed with Paganin phase retrieval, imported into Avizo Lite 9.4 (Thermo Fisher Scientific) and visually explored for mature rachidian teeth that showed no evidence of in vivo wear or post-mortem breaking during sample preparation. The scan volume was then cropped to these sections to facilitate easier data management. Segmentation of one tooth per species was performed semi-automatically: the “Magic Wand” tool was used initially to select the tooth volume based on greyscale intensity and then to manually remove any traces of the supporting radular membrane, mount, or mounting glue remained attached to the teeth. Once segmented, each tooth was exported as an *.stl and brought into Geomagic Wrap 2017 (3D Systems) for smoothing and cleaning of artefacts. In one specimen (Acanthais), damage to the tooth was only apparent after segmentation.

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Specimens were scanned using single propagation distance tomography at the European Synchrotron Radiation Facility (ESRF) in Grenoble, France using the ID19 microtomography beamline. Every specimen used the following scan parameters: 2999 projections collected for a 360° rotation; 0.1 ms exposure time; 360 nm voxel size; 20 mm sample-to-detector distance; x-ray energy of 26.5 keV. Specimen preparation for scanning electron microscopy (SEM) was the same as for synchrotron preparation except that radulae were mounted on stubs with double-sided conductive tape, and coated with Au-Pd for imaging with a Philips XL 30 scanning electron microscope at the Museo Argentino de Ciencias Naturales. Synchrotron *.tif stacks were reconstructed with Paganin phase retrieval, imported into Avizo Lite 9.4 (Thermo Fisher Scientific) and visually explored for mature rachidian teeth that showed no evidence of in vivo wear or post-mortem breaking during sample preparation. The scan volume was then cropped to these sections to facilitate easier data management. Segmentation of one tooth per species was performed semi-automatically: the “Magic Wand” tool was used initially to select the tooth volume based on greyscale intensity and then to manually remove any traces of the supporting radular membrane, mount, or mounting glue remained attached to the teeth. Once segmented, each tooth was exported as an *.stl and brought into Geomagic Wrap 2017 (3D Systems) for smoothing and cleaning of artefacts. In one specimen (Acanthais), damage to the tooth was only apparent after segmentation.

Institutions

University of South Florida

Categories

Evolutionary Biology

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