Kidney injury primes liver ferroptosis
Description
## Study design Male C57BL/6J mice (*Mus musculus*, 10–12 weeks, 25 ± 3 g; The Jackson Laboratory stock #000664) were assigned at random to sham or acute kidney injury, **n = 5 per group**, with the individual animal as the experimental unit and no animal excluded from any analysis. Injury was induced under isoflurane anesthesia by clamping both renal pedicles for **30 min**, core temperature held at 36.8–37.2 °C; sham animals underwent the identical procedure without occlusion. A single reperfusion interval of **24 h** was studied. The liver was perfused in situ with ice-cold phosphate-buffered saline before collection. Sample identifiers are `Sham_1`–`Sham_5` and `AKI_1`–`AKI_5` throughout, one per animal. ## Assays **Bulk hepatic RNA sequencing.** Total RNA extracted in TRIzol, column-purified; library preparation and sequencing performed commercially by GENEWIZ (Azenta Life Sciences). Differential expression by DESeq2 across 13,294 genes retained after filtering. **Targeted oxylipin lipidomics.** 10–20 mg liver spiked with an internal standard mixture, extracted in methanol, measured on a Sciex 6500+ triple-quadrupole mass spectrometer. 57 analytes: five parent polyunsaturated fatty acids and their cyclooxygenase, lipoxygenase and cytochrome P450 products, including specialized pro-resolving mediators. **Multi-omics integration.** Transcriptome and liver proteome integrated by sparse multi-block partial least squares discriminant analysis (DIABLO) in the mixOmics R package, two components, fixed random seed.
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Institutions
- University of South FloridaFlorida, Tampa